Determination of Polyamine Cationic Lipids by LC-MS/MS in SD Rat and Dog Plasma

Polyamine cationic lipids, as nonviral gene delivery agents, have three essential domains: a positive charged polar head group (an amine or polyamine), a linker (ether, ester, or carbamate), and a lipophilic tail (cholesterol or long-chain fatty acid). Usually nucleic acids was wrapped through positively charged electrostatic action on the surface, forming nucleic acid-liposomes. 

This structure make it prone to non-specific binding with surfaces such as plypropylene vials, 96-well plates, pipette tips, autosampler injection syringe, LC tubing, column solid phase, and protein in biological matrix. This can result in a negative bias for calibration standards, quality contra and unknown samples, tailing peak shape, massive carryover, and low recovery when using regular protein precipitation for sample preparation. In the study, a qualified, rapid, and reproducible LC-MS/MS method was developed for the quantitative determination of polyamine cationic lipids in pharmacokinetics study.